Evaluation of In vitro and In vivo anti-alzheimer potential of iminodiasetic acid

Alzheimer's disease (AD) is one of the most common neurodegenerative diseases and accounts for more than 80% of the worldwide population of dementia in elderly people. AD is pathologically characterized by the accumulation of intracellular neurofibrillar tangles and extracellular amyloid protein. Yet, there is no fully qualified treatment for AD. For this reason, therapeutic studies in the areas of AD prevention or slowing down are of great importance. In the study, the anti-Alzhemer potential of iminodiacetic acid (IDA) amino acid was investigated. The potential neuroprotective effect of IDA amino acid, WST-8 test, LDH test and metal chelation study with SH-SY5Y cell line and also biochemical, hematological, histopathological evaluations and molecular genetic analyzes were determined in rats induced experimentally with ALCl3 exposure. Four groups of female Sprague-Dawley rats were used in the study. These groups were as; Group 1 control (n = 4), Group 2 AICI3 exposed experimental AH model group (n = 4), Group 3 IDA (n = 4) and IDA 's neuroprotective effect on AD Group 4 AICI3 + IDA (n=4). Oxidative stress and neurodegeneration were observed in AD-induced rats with AlCl3 exposure. Molecular genetic analysis revealed that aluminum reduces β-secretase and γ-secretase activity while reducing α-secretase activity. Our results revealed that the application of IDA amino acid could reduce the levels of neurodegeneration and oxidative damage caused by aluminum toxicity. We believe that the data obtained from this thesis will give a different perspective to the synthesis of new compounds with AlCl3-dependent neurodegeneration and oxidative stress, and to new treatment strategy researches.