Vibrio infections and treatment showing in cultured sea bass (Dicentrarchus labrax, L. 1758) in the black sea region

In this study, identification of Vibrio species isolated from bacterial infections in cultured sea bass (Dicentrarchus labrax L., 1758) in the Black Sea was performed by using conventional methods, and API 20E test kits. All isolates were confirmed by PCR assays specific to the 16S rRNA gene of bacterium. According to the result of biochemical and PCR tests isolated bacteria determined as V. anguillarum, V. vulnificus, V. parahaemolyticus and V. harveyi. API 20E profile for V. anguillarum isolates was usually determined as 3207526. The sequenced all isolates except for two were found to be similar to Vibrio species at the rate of 98-100% in GenBank under different accession numbers. In addition, in the treatment for vibriosis, it was intended to detect the most effective agents. Results of the testing susceptibility to antibiotics showed that Vibrio isolates were resistant to 94,23 % ampicillin, 42,31 % streptomycin, 13,46 % oxytetracycline and 3,85 % erythromycin, but all strains except two were found susceptible to sulphamethoxazole, trimethoprim-sulfamethoxazole, oxolinic acid, enrofloxacin and florfenicol. According to the results of the susceptibility test, trimethoprim-sulfamethoxazole florfenicol and enrofloxacin were suggested as the most effective chemotherapeutics for vibriosis treatment.