Determination of genetic diversity in Kırklareli honey beepopulations (Apis mellifera L.) by PCR-RFLP analysis in mtDNA COI and ND5 gene regions

In this study, honey bees representing Kırklareli honey bee populations were investigated based on COI and ND5 gene regions in the mitochondrial genome. Restriction fragment length polymorphism (RFLP) method was used to define the Apis mellifera populations. In this study, honey bee populations in the Kırklareli province of Turkey were investigated via COI and ND5 gene regions by PCR-RFLP method using NcoI, SspI, StyI, AluI, HincII, FokI restriction enzymes. A total of 117 worker bee samples were used as material from Kırklareli and Tekirdağ region. In this study, different variation and point mutation was determined as a result of cutting the COI gene region with the NcoI enzyme. The DNA sequence of CCATGG as a result of the G→A transition in the COI/NcoI segment formed a new restriction site as CCATGA and this haplotype was determined as the C haplotype. As a result of the COI/SspI restriction, the C haplotype previously reported in the literature was determined. As a result of the treatment with COI/StyI enzyme, the cut-off point previously reported in the literature was determined from the mutation of the G→A transition and the CCWWGG DNA sequence changed as CCWWGA, and this profile was expressed as the B haplotype. C haplotype was obtained in all the samples as a result of restricting with ND5/AluI restriction enzyme. No digestion was found in all of the populations analyzed with ND5/FokI and ND5/HincII enzymes, and only B haplotype was observed.