Determination of HlA tissue type and investigation of antibody levels according to the etiology of chronic renal failure patients

Objective: The aim of this study was to investigate the association of HLA allele distribution with etiologic subtypes in 1079 patients with chronic kidney disease and 1111 healthy controls. It was also aimed to investigate the association of HLA alleles with the presence of anti-HLA antibodies known to cause graft failure and rejection development. Method: HLA-A, B, DRB1 allels were determined (PRA) by PCR-SSOP method and Luminex (Gen-Probe) LIFECODES HLA-A, B, DR kits. PRA TEST for the detection of G (IgG) antibodies against HLA class I and class II antigens was performed by the luminex method which was designed as A bead-based immunoassay. For statistical analysis, arlequin v3.5.1.2 was used. Results: Statistical data suggest that the etiologic subgroups of patients with CKD HLA-A*01 (p=0.0002), HLA-A*03 (p=0.003), HLA-A*11 (p=0.0007), HLA-B*35 (p=0.001), HLA-B*27 (p=0.001), HLA-B*57 (p=0.005), HLA-DRB1*01(p=0.001), HLA-DRB1*04 (p=0.009), HLA-DRB1*14 (p=0.001) alleles are positively associated with disease, HLA-A*02 (p=0.001), HLA-A*24 (p=0.0001), HLA-A*26 (p=0.0001), HLA-B*07 (p=0.001), HLA-B*08 (p=0.003), HLA-B*51 (p=0.001), HLA-DRB1*13 (p=0.001), DRB1*11 (p=0.001), HLA-DRB1*03 (p=0.001) alleles were found to be negatively related. Different results were obtained in the etiologic subtypes of the disease. Conclusion: HLA alleles with positive or negative association with the disease from our study results will contribute to the literature. Shedding light to the relationship between PRA positivity and HLA alles will have an importamt role in transplant success.