Investigation of phytoplasma diseases in the pear production areas of Malatya province by molecular methods

In order to determine the phytoplasma diseases in the pear production areas of Malatya province, survey studies were carried out in 2020, and a total of 301 symptomatic or non symptomatic leaf samples, were randomly collected from pear plantations of Malatya province. Total DNA isolations from the collected samples were performed using a commercial kit. The presence of phytoplasma agents in the collected samples was investigated using the Nested polymerase chain reaction (Nested PCR) method using universal primers: R16mF2/R16mR1 and R16F2n/R16R2. In the study, No phytoplasma agent was not detected in the samples collected from the pear production areas of Malatya province.