Investigation of polymorphism in some native cattles for lactoferri̇n gene providing resistance to masti̇ti̇s

This study was carried out to determine Laktoferrin gene polymorphism in Native cattle raised in Aydın province. DNA was extracted from 100 individual blood samples collected from cattle raised in 6 flocks at different locations. The PCR-RFLP methods were used to determine genotypes. A 1050 bp DNA fragment within bovine Laktoferrin gene was amplified with PCR and then digested with restriction endonuclease enzyme HinfI. The HinfI digestion produced a mixture containing of 635, 480, 180 and 120 bp. In this study we have detected a new allele as ''C'' with different fragments (480, 180 and 120 bp) which has not been observed in literature yet. In present study, A and C allele frequencies were identified with 0.435 and 0.565; AA, AC and CC genotype frequencies were identified with 0.17, 0.53 and 0.30, respectively. The B allele of Laktoferrin gene has not been observed. In order to reveal more concrete information, sequencing for the Laktoferrin gene may be useful. Results obtained from this study will provide a significant contribution to the literature.