Botanical properties of mentha longifolia (l.) hudson subsp. longifolia (l.) hudson and mentha longifolia (l.) hudson subsp. typhoides (briq.) harley var. typhoides (l.) hudson, chemical contents and analgesic activities of their leaves extracts

BOTANICAL PROPERTIES OF MENTHA LONGIFOLIA (L.) HUDSON SUBSP. LONGIFOLIA (L.) HUDSON AND MENTHA LONGIFOLIA (L.) HUDSON SUBSP. TYPHOIDES (BRIQ.) HARLEY VAR. TYPHOIDES (L.) HUDSON, CHEMICAL CONTENTS AND ANALGESIC ACTIVITIES OF THEIR LEAVES EXTRACTS The leaves, flowers, stems and seeds of Mentha longifolia L., which are used in the pharmaceutical, food and cosmetic industries, are used in the digestion problems and gastric spasms among the public. In our study; The botanical characteristics of the Mentha longifolia (L.) Hudson subsp. longifolia (L.) Hudson and Mentha longifolia (L.) Hudson subsp. typhoides (Briq.) Harley var. typhoides (L.) Hudson, where collected from Uzundere in Kümbet Village of Dereli District of Giresun, have been established and the volatile oil contents of both taxa have been determined by GC-MS. Main components of the first taxon are menton, isomentone, pulegon and for the second taxon are menton, isomenthone, pulegone, cis-piperitoneoxide were determined. In addition, water and methanol extracts of these two subspecies were obtained by microwave, soxhlet and ultrasonic bath methods. Total phenol and flavonoid amounts of the extracts were carried out by UV-Vis spectrophotometer device and phenolic compounds, flavonoids of extracts were carried out by UHPLC device. When the methods used in extraction are compared; it was determined that the highest phenol content of extract from ultrasonic extraction. Phenolic contents of methanolic extracts were found higher than aqueous extracts (177.6±0.04 mg/g). Rate of phenolic contents of Mentha longifolia subsp. typhoides var. typhoides (TYP) was found that higher than Mentha longifolia subsp. longifolia (LONG). The analgesic effects of methanol extracts, were determined by formalin test in the first stage. After the most effective extract was found. The antinociceptive effect of the highest efficacious extract (TYP methanol extracts) was determined by using hot plate test. Lorke method was used for acute toxicity test. No deaths were observed in both TYP and LONG extracts after 48 hours. As a result, LD50 was determined to be >3200 mg/kg. Key words: Mentha longifolia, Phenol, Analgesic activity