Development of SSR (simple sequence repeat) markers in lentil

In this study, genomic libraries that were enriched with four different microsatellite motifs (CA, GA, AAC, and ATG) were developed by using genomic DNA of Karacadağ genotype. From these genomic libraries, total 432 clones were sequenced. Out of the 432 clones, 110 clones eliminated because some of these didn?t contain SSR repeats or had same duplicated sequences. Total 360 SSR regions were detected and primer pairs were designed for these SSR regions, from the remaining 322 clones. Optimum annealing temperature was determined for 220 out of 360 primer pairs. 149 primers pairs, which have a known optimum annealing temperature, were used for genetic diversity analysis in Turkish lentil cultivars. Out of the 149 primers, 71 primer pairs amplified monomorphic fragments and 78 primer pairs produced 400 polymorphic alleles in 15 lentil cultivars. The average number of allele per locus was 5.1 and the polymorphism information content (PIC) of primers ranged from 0.06 to 0.89 with an average of 0.58. The UPGMA based dendogram separated all lentil cultivars into two main groups. Using dendogram analysis, it will be possible to select genotypes which should be used for constructing map population and development of new cultivars for lentil. This developed SSR markers can be used in the studies such as development of present genetic maps in QTL, MAS and genetic diversity in lentil.