Comparison of the activities of glutathione S-transferase isozymes and total thiol content of gastric tissues obtained from obesity patients by gastrectomy operation

Obesity has become an important public health problem in many countries because of its increasing prevalence, its relation with many diseases and mortality. Studies have shown that obesity is associated with oxidative stress. In this study, the effect of obesity and related diseases on GSH molecule and GST enzyme which are important members of endogenous defense mechanism were investigated. The samples of gastric tissue which was taken from 39 obese patients, were homogenized. GSH levels were analyzed. GST enzyme activity was measured towards CDNB, pNBC, EPNP substrates in the ELISA Microplate Reader system. Patients were grouped according to gender, age, hypertension, diabetes and family history. In the relationship of BMI-GSH-Total GST activity, GSH was found to be relatively low when total GST activity was high. In the relationship between age-GSH and total GST, GSH level and total GST activity increased with age. In comparison with gender, the mean values of all activity values measured in three different substrates averages of female patients were found higher than the averages of males. The present study is the first study to compare activities of GST isozymes and total thiol content in the stomach tissue of obese patients in Turkish population grouped according to age, gender, hypertension case, diabetes case and family history parameters. This study was conducted with the approval (No: 2012-KAEK-15 / (1678) 1800) of Ankara Kecioren Training and Research Hospital Medical Research Ethics Committee.