Pentoxyphiline and platelet acti̇vati̇ng faktor sperm chromati̇n structure of dna damage distribution method

Approximately 15 percent of married couples have infertility problems. 30-50% of infertility cases are due to the reasons related to men. One of the main causes of male infertility is the disorder in the sperm motility. A variety of agents have been identified to improve sperm motility's of men who have sperm motility problems. These factors are the basic requirements in the initiation and continuation of motility. cAMP plays a key role in the regulation of sperm function. The basis of the stimulation of sperm motility in vitro conditions is based on the stimulation of sperm functions by increasing intracellular cAMP levels. When phosphodiesterase inhibitors, such as pentoxifylline, are added to semen in the laboratory, they increase intracellular cAMP level, glucose and the production of ATP. These substances increase the rate of motile sperm and at the same time they start lively but nonmotilspermatozoa motility. It has also been observed that it stimulates frozen-dissolved sperm motility. PAF is present in human sperm and endogenous content has an important and positive relationship between motility and the rate of pregnancy. Although the exact mechanism of action of PAF isn't certain, its function about normal reproduction is obvious. Endogenous PAF may also play a role as a biomarker for normal sperm function. One of the special advantage of using PAF is because of the fact that it is a natural substance produced by sperm and it is non-toxic unlike some other motility stimulants such as pentoxifylline. Improving sperm motility can efficiently increase the results of assisted reproduction techniques. In this project, we aimed to analyse the DNA damage with Sperm Chromatin Distribution (SCD) method after the application of Pentoxifylline and Platelet Activating Factor(PAF).