Dynamic and complexation study of hydroxy amide derivative based on pyridine with some organic amine salts and T1 and T2 relaxation time measurements by nmr spectrometer

In this study; The dynamic features of Pyridine-based β-hydroxy amide 1 and β-hydroxy amide 2 which are chiral amino alcohol derivatives and their interaction with D- and L-phenylalanine methyl ester hydrochloride amine salt was examined using Bruker 400 MHz NMR spectrometer. Spin-lattice (T1) and spin-spin (T2) relaxation times of Pyridine-based β-hydroxy amide 1 and 2 were measured at room temperature (25 ℃) by NMR spectrometer. The correlation time(τ)derived from the ratio of the (1/T1)/(1/T2) using relaxation formulas pertaining to dipolar interactions was calculated for each peak The τ values of β-Hydroxy amide-1 ranged from 9,18x10-11s- 1,42x10-9s while β-Hydroxy amide -2 ranged from 3,37x10-10s-2,87x10-9s The similarity of τ values of the peaks belonging to the different parts of the molecule suggested that overall molecular tumbling may be responsible for relaxation mechanism of all the groups. Molecular recognisition properties of Pyridine-based β-hydroxy amide -1 and -2 towards the D-PheOMe.HCl and L-PheOMe.HCl amine salts prepared at various concentrations of host-guest solutions were investigated by (_ ^1)H NMR titration method. Association costants were found from chemical shifts.It was concluded that each ligand binding better and highest enantioselectiviyt to L-enantiomer compared to D-enantiomer.