Antioxidant effects of rutin and chrysin composited with polyethylene glycol on saccharomyces cerevisiae L exposed to 7, 12-dimethylbenz[a]anthracene

In this study, the metabolic effects of flavonoid components such as Rutin and Chrysin against 7,12-Dimethylbenz[a]anthracene (DMBA) were investigated on S. cerevisiae by forming composites with water-soluble structures such as polyethylene glycol (PEG). For this purpose, changes in antioxidant enzymes, fatty acids, lipid peroxidation and lipophilic molecules in the cell were analyzed. The experimental planning was made as control, DMBA, Rutin, Chrysin, PEG, Sesame oil, DMSO and combination groups. DMBA, known as 7,12-Dimethylbenz[a]anthracene, is a carcinogenic agent and is widely used in both cellular and experimental animal models. According to our experimental results, it was observed that the total protein amount, MDA, GSH and GSSG levels increased in the routine given experimental model, but it was found to decrease only in the routine given group. In the experiment group where Chrysin was given, it was determined that these results increased at different rates in the PEG, sesame oil and combination groups. It was determined that the activities of antioxidant enzymes such as SOD, CAT, GSHpx and GST decreased in all routine groups compared to the control group. A decrease in CAT and GST results was also observed in the chrysin-applied groups. It was determined that the amount of palmitic acid, the end product of fatty acid synthetase enzyme (FAS), decreased in the routine-applied groups of DMBA, sesame oil and composite groups and increased in the other groups. Similar results were observed in the same experimental groups where chrysin was applied. While the amount of ergosterol from lipophilic molecules increased significantly in the DMBA group of the routine-applied model, it decreased in the other groups. In the chrysin-applied model, an increase was observed in the DMBA, chrysin and PEG groups. As a result, it was concluded that the routine and chrysin added to the culture medium and other molecules and composites were effective on the activity of antioxidant enzymes in S. cerevisiae, lipid peroxidation, FAS and stearol CoA enzyme products.