Cryopreservation of primary rabbit kidney cell culture

This study was performed for investigation the factors associated with propagation of primary rabbit kidney cell culture and to optimize cryopreservation of the cells. When the factors (Na+, K+, 02, C02, H+, pH, cell viability rate, cell count) related with growth of cells were evaluated, it was observed that Hanks' medium with 10% fetal bovine serum was appropriate for maintenance of primary rabbit kidney cell culture. It was detected that the best method for the cryopreservation of cells was the method with which the cells in the freezing medium containing fetal bovine serum and Hanks' medium (1:1) and % 10 DMSO were holded at +4°C for 30 minutes, then at -20CC for 1 hour and at -70°C overnight in a styrofoam box and stored in liquid nitrogen for 40 days. The cell viability was found to be 84% with this method. Key Words: Primary rabbit kidney cell culture, Cryopreservation, Hanks' medium. Na+, K+, 02, C02, H+.