The investigation of the effect of Ellagic acid application in bone healing in the rat calvarial critical size defect

This research aims to stimulate osteogenesis with graft materials placed on bone tissue and use its osteoconductive feature; and to evaluate histopathologically the effect of ellagic acid on osteogenesis by increasing osteoblast cells. In this research, general anesthesia of totally 80 Wistar rats were provided with 8 mg/100g intra-muscular ketamine and the rats were divided into 4 groups randomly. 1. Control Group: Calvarial bone defect was created on 20 rats, the defect was left empty without any intervention and the wound area was sutured. 10 of the rats were sacrificed at the end of 4th week and the rest 10 rats were sacrificed at the end of 8th week. 2. Experimental Group: 8mm- calvarial bone defect was created on 20 rats, the defect was left empty without any intervention and the wound area was sutured. Ellagic Acid was orally administered to rats for 4 weeks, by means of a gastric tube 50mg/kg body weight/day. 10 of the rats were sacrificed at the end of 4th week and the rest 10 rats were sacrificed at the end of 8th week. 3. Experimental Group: 8mm-calvarial bone defect was created on 20 rats. Alloplastic bone graft was treated on prepared defect. 10 of the rats were sacrificed at the end of the 4th week and the rest 10 rats were sacrificed at the end of 8th week. 4. Experimental Group: 8mm-calvarial bone defect was created on 20 rats. Alloplastic bone graft was treated on prepared defect. Ellagic Acid was orally administered to rats for 4 weeks, by means of a gastric tube 50mg/kg body weight/day. 10 of the rats were sacrificed at the end of the 4th week and the rest 10 rats were sacrificed at the end of 8th week. XI Specimens obtained at the end of the research were evaluated histopathologically at Dicle University, Medicine Faculty, the laboratory of Histology Department. Obtained results were assessed using Kruskal-Wallis and Mann-Whitney U tests. When the research which was conducted considering different parameters is evaluated, it is thought that ellagic acid is not superior to graft in terms of accelerating osteogenesis. When the results of 4 th and 8 th weeks are evaluated, the best results are seen in graft+ ellagic acid group in terms of new bone formation. It can be said that the treatment of ellagic acid by its own increases osteoblast cells and directly increases osteogenesis. Anahtar Kelimeler: 1. Ellagic Acid 2. Bone Healing 3. Calvarial Defect 4.Antioxidant