Genetic mapping of Citrus maxima (Burn) Merr x (C. maxima (Burn) Merr. x Citrus clementina hort. ex. Tanaka) backcross population by using SSR markers

Citrus family has the great importance around world and Turkey considering producer amount, production area,scale and production amount. Genetic mapping, identification of the relations of individuals in a popoulation and grouping the genetic relations are being considerd as important tools for Molecular Plant Breeding. The backcross population of Citrus maxima (Burn) Merr. x (C. maxima (Burn) Merr. x Citrus clementina hort. ex. Tanaka) provided by CIRAD France was used as a material in this study. In this study the codominant SSR technique was choosen considering its? high polimorphism, reproducibility and the availability of having more information comparing other techniques. In this study, 149 SSR primers were analysed. Out of 149 primes parentally polimorfic ones were used for all individuals of population. The DNA profiles were evaluated. The profiles were tested via chi square test on the basis of compatibility of Mendels? segregation. The SSR data matching with Mendels? segregation were evaluated with the software JOINMAP 2.0. As a result out of 47 SSR primers, 32 were used for Genetic mapping. The Map consists of 8 linkage groups with total lenght; 293,9 cM and the average genetic distance between two markers is 8,6 cM.