An experimental study in a rat model: The research of the effect of mesenchymal stem cells derived from autogenous fat tissue to the capsule formation around silicone implants

In this study, the effect of mesenchymal stem cells that derived from autogenous fat tissue to the capsule formation around silicone implants has been searched.16 Wistar Albino rats, weight of 270-310 gr, were used for the study. The subjects are divided into equal groups as experiment group (Group II) and control group (Group I). Subcutaneous silicone implants was placed in the pectoral area of all rats in both groups by opening a pouch for implant. While it was not used an additional therapy for Group I, it was injected a suspension containing 40 million cells / cc to the pouch where the silicone implant placed in Group II. All implants excised at the postoperative 8th week from both groups. Capsule formation removed totally and took histopathological examination.Samples were examined by histology specialist: Morphometric evaluation with volumetric measurement of capsules thickness, evaluation of the amount of collagen-procollagen, light microscopy with Masson Trichrome dyeing. In morphometric evaluation of capsule thickness there was a significant difference between Group I and Group II (p<0,032). In Group II the amount of procollagen- collagen was lower than Group I. In light microscopy neoangiogenesis in Group II higher than Group I.In this study it is founded that the use of 40 million cells/cc mesenchymal stem cells reduce the capsule formation around the silicone implant and the silicone implant does not form foreign body reaction.