Immobilization technique in the cell cultures of black henbane (hyoscyamus niger) and increasing the production of phenolic compounds with tropan alkaloids with external applications

This study was carried out to determine the effects of 24-epibrassinolide (24-EBL) and methyl jasmonate (MeJA) applications on the cell growth and secondary metabolite accumulation of immobilized cells in black henbane (Hyoscyamus niger). For this purpose, different concentrations of 24-eBL (0.5, 1 and 2 mg/l) were applied to immobilized cells covered calcium alginate beads alone or the combination of 1 mM MeJA for 30 days. After harvest, immobilized cells were evaluated for fresh cell weight, dry cell weight, cell growth index, contents of tropane alkaloids including hyoscyamine and scopolamine and phenolics compounds. As a result of this study, 2 mg/l 24-eBL was the most suitable application providing the highest cell growth parameters while 0.5 mg/l of 24-eBL for scopolamine and hyoscyamine. On the other hand, 1 mM MeJA with 24-eBL gave the maximum values for phenolics. Consequently, it was determined that 24-eBL and MeJA treatments can be used successfully in order to increase the production of tropane alkaloids and phenolics compounds in immobilized cells of black henbane.