An investigation of the effect of black tea extract on the enzyme carbonic anhydrase

Tea is obtained from a plant known as Camellia sinensis. It is a traditional beverage consumed at any time of day by Turkish people. Today, the worldwide production of black tea, green and oolong varieties includes 3 there. Carbonic anhydrase (E.C. 4. 2. 1. 1), is a metaloenzyme, containing Zn+2 in the center. The substrate of this enzyme is CO2 in vertebrate. This enzyme catalyzes conversion of CO2 to H2CO3 in cells and in intercellular fluid.In this study the inhibitory effect of tea on the enzyme carbonic anhydrase were investigated.Firstly, erythrocyte carbonic anhydrase isoenzymes (CA-I, CA-II) from human erythrocyte were separately purifed by affinity chromatography.Then different concentration of tea on the human erythrocyte CA were analyzed. In the studies, it was taken advantage of hydrates and esterase activites for determining of carbonic anhydrase activitates. It was observed that different concentration showed inhibition effect on human erythrocyte CA hydratase and esterase activity. % Activity indicates the effect of inhibition for tea- inhibitory concentration [I] graphs are plotted.According to data obtained in this study, lower concentrations of tea showed inhibitory effect, higher concentrations showed activating effects.Key Words: Carbonic Anhydrase, inhibition, affinity chromatography.