Effects of strigolactone and mycorrhiza treatments on the development and nutrient element uptaking of grapevine saplings in 110R/Narince grafting combination

In this study, the effects of different doses of GR24 (a synthetic strigolactone analog) and arbuscular mycorrhizal fungus (AMF) on root and shoot development and nutrient uptake in greenhouse potted sapling production using the 110R/Narince grafting combination were investigated. Grafted cuttings were grafted using the omega section method. After callus development was achieved in the incubation chamber, they were planted in black polyethylene tubes containing perlite and peat (1:1) under greenhouse conditions. Treatments were made in two stages: pre- and post-planting. GR24 (1, 5, and 10 µM) solutions were inoculated for 1 hour and injected with 5 ml of root solution. AMF was inoculated for 1 minute and injected with 30 ml of root solution. Leaf number, shoot length, shoot diameter, shoot fresh and dry weights, total leaf area, nitrogen balance index (NBI), chlorophyll, flavonoid, anthocyanin, stem and root mineral contents, root length, root number, development level, root fresh and dry weights, root dry matter ratio were considered. Results showed that GR24 and AMF treatments provided statistically significant increases in root and shoot development compared to the control group (p<0.05). Specifically, 1 and 5 µM GR24 yielded the highest root and shoot parameters, while the GR24 + AMF combination was prominent in root development. In conclusion, the combined use of strigolactone and mycorrhizal treatments synergistically enhanced morphological development and nutrient uptake in grapevine saplings.