The effect of beta glucan on the experimental colitis model that induced by TNBS

Oxidative stress and inflammation are considerable risk factors in the pathogenesis of inflammatory bowell disease (IBD). There are many studies about the effects of anti-inflamatory and antioxidant of beta glucan (BG), but it has not observed in the form of a study that conducted to investigate the effects of Trinitrobenzen Sulfonic Asid (TNBS)-induced colitis model in the rats. In accordance with this information, it is intended to invastigate the effects of BG through the antioxidant and anti-inflammatory mechanisms in TNBS-induced colitis. In the research, for a total of 32 rats which weights ranging from 250-300 g, divide into 4 experimental groups as sham control (n=8), TNBS (n=8), TNBS-BG+3 (n=8), and TNBS-BG+10 (n=8). Saline was given to the Sham group and TNBS was given to the colitis group with canula as intrarectal under anesthesia. BG was given to the BG+3 3 days after creating colitis dose of 100 mg/kg intrarectally. BG was given to the BG+10 7 days before colitis and 3 days after creating colitis dose of 100 mg/kg intrarectally. After fasting the animals overnight and emptying the colons on the morning of experiment, inflammation was induced in the colon by the intrarectal administration of 0,8 ml of a 25 mg 2,4,6-trinitrobenzen sulfonic acid (TNBS) dissolved in 37 % ethanol using an 8 cm-long cannula under anesthesia. Animals were sacrificed on day 4. after induction of colitis and the last 10 cm of the colon was excised, opened longitudinally. Longitudinal colon segments were subjected to biochemical and histopathological examination. Statistical analyses of data were carried out using the GraphPad program. BG caused a significant decrease in intestinal injury as determined by the biochemical results and histological scores (p<0.05). Tissue malondialdehyde (MDA) levels were higher in the TNBS group than in the Sham and TNBS-BG groups (p<0.05). These values were reduced in the rats treated with BG (p <0.05). Glutathione (GSH) enzyme activity was lower in the TNBS group compared to sham control and TNBS-BG groups. Myeloperoxidase (MPO) enzyme activity was lower in the sham control group compared to TNBS group. Antioxidant enzyme activities were increased by BG treatment compared to TNBS group. While the increase in GSH activity was significant (p<0,05). And any significant change wasnt observed between the groups in the catalase (CAT) enzyme activity. In addition, treatment with BG also reduced elevations in myeloperoxidase (MPO) activity occuring in colonic tissue after induction of colitis. Beta Glucan seems to have anti-inflammatory and antioxidant properties. Therapy with BG carries a potential to reduce the intestinal injury on TNBS-induced colitis in rats.