Investigation of trichomonas vaginalis frequency by microscopy and different culture methods

Trichomonas vaginalis is a flagellated protozoon that is common in the world and affects the urogenital system of the human and causes the disease called trichomoniasis. The purpose of this study is to determine the frequency of Trichomonas vaginalis in women residing in Siirt. The study was carried out on 150 volunteer female patients aged between 18-65 who applied to the Gynecology and Obstetrics and Polyclinics of Siirt State Hospital between June and October 2019. The study was carried out on patients who received voluntary approvals after the procedures were fully described. Parasites were investigated by direct microscopic examination, Cysteine Peptone Liver Maltose (CPLM) and Trichomonas Broth (TB) culture methods in samples taken from the posterior vaginal fornix during pelvic examination. Trophozoit detected sample by any method was evaluated as positive in total. Trichomonas vaginalis infection was detected in 25 (16.6%) of 150 patients. Of 25 patients who were found to be positive, 12 (8%) by direct microscopic examination, 15 (10%) by CPLM culture method and 18 (12%) by TB culture method were positive. By using different diagnostic methods together, skipping of cases not detected by a diagnostic method is prevented. As a result, it was concluded that in addition to direct microscopic methods, one of the higher sensitivity culture methods should be applied in order to ensure the correct diagnosis and treatment of patients with suspected trichomoniasis.