Synthesis, characterization, toxicity and biodistribution of Tryptophan Graft-Poly (HEMA) polymeric nanoparticle

Nanotechnology is a field that has attracted a lot of interest in recent years and has developed many products. Because of the advantages of polymers, their use as biomaterials is remarkable. Polymeric nanoparticles have an important position in nanotechnology with their use in biotechnological and biomedical fields such as disease diagnosis, imaging and therapy. In this thesis study, synthesis, characterization, investigation of in vitro and in vivo toxicity potential and determination of biodistribution of Tryptophan-graft-poly(HEMA) polymeric nanoparticle [Trp-graft-p(HEMA)]. p(HEMA) was synthesized by surfactant free emulsion polymerization method and then Trp-graft-p(HEMA) was obtained by grafting with Trp. In the characterization of the polymeric nanoparticle, FTIR, Zeta potential and size analysis, SEM and swelling test were used. The average particle size is 164.1 nm, the polydispersity index is 0.501 and the surface charge of the nanopolymer is negative -19.6 mV. The amount of swelling of the nanopolymer decreases with increasing temperature. MTT test, hemolysis test, HET-CAM test and AMES test were performed to determine the in vitro toxicity potential of the nanopolymer, and it has been determined that the nanopolymer has no cytotoxic and hemolytic activity, has antiangiogenic effect and should continue its studies in order to determine the genotoxic potential. In vivo toxicity studies conducted with Balb/c albino experimental mice indicated that the nanopolymer did not show toxicity in acute doses. Because of the physicochemical properties of the nanopolymer, the biodistribution study could not be performed because of the non-dispersibility in PBS.