WASL(N-WASP) gen ekspresyonunun pterjiyumdaki etkisi

Pterygium is a benign growth of the conjunctive tissue that covers the sclera with a high proliferation property which causes intrusive ocular surface and wide matrix remodeling. WASL which also known as n-Wasp (Neural Wiskott-Aldrich syndrome protein) is a nucleation-promoting factor that promotes the formation of branching actin filaments. The WASL gene is located on the q arm (long arm) of chromosome 7, which locate q31.32 and encodes a 505 amino acid sequence. The WASL gene comprises 11 exons, and the encoded protein has a molecular weight of about 54.84 kD. The WASL gene has several important molecular function, including a actin binding, GTPase regulator activity. SH3 binding motifs with a proline-rich locus, and the C-terminal acidic region, which plays a role in actin cytoskeleton remodeling by stimulating Arp2/3. The WASL gene appears to play a function in signal transduction as well as cytoskeletal remodeling. Regulation of cortical actin filament rearrangement in response to external stimuli is mediated through the Arp2/3 complex, which links small GTPases (e.g., Rac and Cdc42) with actin polymerization. The aim of this study was to see if the expression of the WASL gene changed in pterygium versus healthy cataract tissue. Tissues were removed with the patient's consent and awareness throughout the procedure. We use the WASL gene expression approach to harvest cDNA from tissue levels determined by real-time PCR. WASL gene expression levels were determined to be statistically significant higher in tissues with pterygium compared to the control group (p < 0.05), based on our findings that the increase in WASL gene expression is hypothesized to be linked to the formation and development of pterygium. According to qRT-PCR data, WASL gene expression is pterygium tissue compared to healthy conjunctive tissue (1.320±0,254-foldchange, p=0.001).