Yeni krem formülasyonları geliştirilmesi ve bu formülasyonların yara iyileşmesi üzerindeki etkilerinin karşılaştırılması

Some of the beneficial ingredients that are traditionally used in wound healing are obtained from some plants, but their effectiveness has not been scientifically evaluated yet. In this study, cream formulations (levant storax and complex creams) with the same cream base (placebo cream) were developed. Addition to the ingredients used in the cream base, levant storax cream contained balsam of oriental sweet gum, while complex cream contained calendula oil, St. John?s wort extract, escin, freeze dried powder of Aloe vera (L.) Burm.f. leaf juice and allantoin. Following the development of cream formulations of levant storax, complex and placebo, the characterization and stability tests were performed at predetermined time and conditions. The stability studies indicated that, physical properties of all formulations were unchanged during the test period of two months. In order to analyse the concentrations of herbal actives, hyperoside and escin, HPLC methods were studied and validated. The validated test methods were also suitable to test concentration of hyperoside and escin in complex cream. The wound healing potential of levant storax and complex creams were tested against a reference cream Madecassol®, negative control and placebo cream by employing an in-vivo excision wound model on rats. The studies indicated that levant storax cream treated rats had the best healing rates compared to all the other groups, whereas the group that was treated with complex cream showed a better healing rate than control and placebo groups. However no significant difference was found between the complex and the reference groups.