Production of bioactive added transdermal tissue skeleton using 3D bioprinting method and mathematical modeling of release kinetics

The field of tissue engineering is a way to help the body regain lost skin in cases where the self-healing process is impossible or inadequate, such as skin infections, injuries and burn problems. Artificial skin production in the treatment of skin infections is provided by bioprinting devices. In order to determine the optimum PVA/CS-alginate/ Turmeric composition for bioprinting scaffold, PVA/CS-alginate mixture of different compositions was used to investigate the microstructure, physicochemical properties and printability of hydrogels. In this study, we synthesised nanocomposites from the combination of chitosan (KS), sodium alginate (ALG) and polyvinyl alcohol (PVA). Then, turmeric was encapsulated into the nanocomposites and characterised by Fourier-transform infrared spectroscopy (FTIR), electron microscopy (SEM). In addition to these studies, the release kinetics mechanism of turmeric bioactive substance was measured by Franz diffusion cell and mathematical models were analysed. In addition, encapsulated turmeric showed higher antibacterial, antioxidant and anti-inflammatory activity than free turmeric. According to the results, the maximum inhibition for albumin denaturation (83.59%), maximum antioxidant activity (85.79%) and the highest TPC content with 23.68 mg GAE/100 g were found in PVA/CS-ALG biocomposite. Therefore, it was concluded that the simultaneous use of three biopolymers KS and ALG and PVA would synergistically enhance the therapeutic efficacy of turmeric. As a result, the new nanocomposite of PVA/KS-ALG biocomposite can be used to treat various diseases, especially inflammatory diseases and cancer.