Investigation of the clinical effects of TLR4 genetic variant in children with adenoid hypertrophy

In our study,the frequency of the TLR4 gene polymorphismsAsp299Gly (rs4986790) and Thr399Ile (rs4986791) polymorphisms in children aged 2-15 years diagnosed with Adenoid Hypertrophy (AH)and the relationship of these variants with the clinical findings of the diseasewas investigated. The study included a total of 192 volunteers: 47 girls and 49 boys, for a total of 96 children diagnosed with AH, and 45girls and 51 boys, for a total of 96 children in the control group who were not diagnosed with AH. The AH diagnosis was based on the evaluation of an ENT specialistand endoscopic findings. For genetic analysis, DNA was isolated from blood samples and the TLR4 Asp299Gly and Thr399Ile polymorphisms were determined using the PCR-RFLP method. The obtained genotypedistributions were statistically evaluated using the Fisher-Freeman-Halton Exact Test, allele frequencies, and Fisher's Exact Test. Furthermore, the clinical characteristics of AH patients were correlated with genotypes. For TLR4 Asp299Gly, in the AHgroup, AA: 87.5% (n=84), AG: 7.3% (n=7), GG: 5.2% (n=5), and in the control group,AA: 94.8% (n=91), AG: 5.4% (n=5), GG: 0% (p=1.000). For the TLR4Thr399Ile variant, the patient group had CC (89.6%, n=86), CT (6.3%, n=6), and TT(4.2%, n=4), while in the control group, CC (96.9%, n=93), CT (3.1%, n=3), and TT (0.n=0) were found. (p=1.000). The TLR4 Asp299Gly and Thr399Ile allele frequencies showed statistically significant differencescompared to the control group (p< 0.05).