Anter resistant culture by nematodes si̇vri̇, green, stuffed and kapy pepper development of pure lines

This study was carried out in the laboratories and greenhouses of Department of Horticulture Faculty of Agriculture Cukurova University, Alata Horticultural Research Institute and Troya Seed Company between 2015 and 2016. The pure lines were obtained from F1 plants via anther culture methods. F1 plants were obtained via crossing of three nematode resistant pepper genitors obtained from the studies carried out at the Alata Horticultural Research Institute with breeding pepper lines of Troya Seed Company selected in terms of high fruit quality and desired agronomic characters. MS medium contained 30 g/l sucrose, 0,25% activated charcoal, +15 mg/l AgNO3, 4 mg/l NAA and 0,5 mg/l BAP was used as nutrient medium. The cultivated anthers were transferred hormone free MS nutrient medium after 35 days. The anther percentage produced embryo, the embryo percentage, the embryo percentage produced plant, the developed plant percentage and the haploid plant percentage were recorded in the study. As a result of the experiment; it was observed that different parameters were changed according to genotypes. The highest embryo yield was obtained from the genotype numbered Z-tad 87 (Nematode resistance x bell hybrid). Among nematode resistance x bell, nematode resistance x green long, nematode resistance x charleston and nematode resistance x capia; bell pepper type gave the highest embryo yield and this was followed by capia, charleston and green long types. The embryo percentage produced plant was observed in green long pepper type. The highest haploid plant percentage was observed in capia pepper type, this was followed by charleston. As a result, all genotypes tested reacted to anther culture and the number of embryo and haploid plants obtained were varied. In the study, three homozygote pure lines contained Me1, Me3; N ve Me7 resistance genes were recorded. Also, one homozygote pure lines contained Me1 was noted. These obtained genotypes resistant to nematode can also be used in breeding studies.