The effect of some phosphazenes with aromatic cyclic on unsaturated fatty acids and ergosterol synthesis in saccharomyces cerevisiae l. culture

In this study, some aromatic on Saccharomyces cerevisiae L. using some cyclic phosphazenes, their effect on ergosterol and unsaturated fatty acids was determined. Different phosphazene molecules were used in the study. The experimental groups within the scope of the study were organized as control group, H2O2 (Hydrogen peroxide) and groups of phosphazen molecules. After the groups were prepared, 30 µg of phosphazen and 100 µl of H2O2 were added to other cultures, except for the control group of S. cerevisiae culture. Incubated for 72 hours in the oven at 30 °C. At the end of incubation, cell pellets were separated and Tris-ETDA buffer homogenized. Total protein levels were determined from Glutathione S-Transferase (GST) from this homogenizat. The fatty acid and lipophilic molecules were analyzed from the homogenate obtained with the hexane / isopropanol alcohol mixture. According to our experimental results, while total protein values and GST values increased in parallel with the phosphazen molecule and H2O2 added yeast cells, GST levels were decreased in some groups, although an increase in the amount of protein was observed. Ergosterol, which has an important place in the membrane structure of S. cerevisiae, was found to be higher in T3 and T3B coded phosphazenes and H2O2 groups compared to the control group, and low in T4 coded phosphazenes and H2O2 groups. It was observed that high levels of oleic acid and palmitoleic acids were among the unsaturated fatty acids in the S. cerevisiae fatty acid composition. When the ratios of these fatty acids were compared between the groups, it was observed that phosphazenes had both a decreasing and increasing effect on the fatty acid synthesis compared to the molecular groups. It was also determined that groups that added hydrogen peroxide had an inducing effect on the fatty acid levels. Our study results revealed that as a result of the addition of phosphazen and H2O2 molecules to the culture medium of S. cerevisiae, ergosterol and fatty acid synthesis, fatty acids cause increases or decreases in the end products of enzymes that double-enter the hydrocarbon chain. Similar effects were observed in cell pellets of culture media where phosphazen molecules were added alone