The investigation of bacteri flora in digestion system of some members of Curculionidae (Coleoptera) family

In this study, the bacteria flora in digestive system of 5 species (Curculio nucum (Linnaeus, 1758); Eusomus ovulum Germar, 1824; Hypera postica Gyllenhal, 1813; Lixus cardui Olivier, 1808; Sitona pucticollis Stephens, 1831) of Curculionidae (Coleoptera) were investigated. The samples were collected from different localities in Kırşehir city between May and August in 2014 year. The catch samples put in steril tube and lively bring to laboratuary. Samples dissected and removed to digestive system, and spreaded to plaque of nutrient agar at the steril conditions. Developed single colonies selected and purified and achieved 17 isolates. The isolates were described by morphologic, physiological, biochemical tests and method of molecular methods ( 16S rRNA PCR and 16S rRNA sequence analysis). As a result of assessment which datums of phenotypic and genotypic characterization and identified of 17 isolates were made and 11 different bacterial species (Bacillus amyloliquefaciens, Bacillus pumilus, Bacillus subtilis, Enterobacter cloacae, Escherichia coli, Klebsiella pneumonia, Lactococcus garviae, Stenotrophomonas maltophilia, Stenotrophomonas rhizophila, Pantoea agglomerans ve Pseudomonas trivialis) determined. As a result, the bacterial species that achieved from the spesies of Curculionidae family which have economic and ecological importance can used.