Determination of heritability of zinc-efficiency in pepper (Capsicum annuum L.) and development of molecular markers linked to qtl/genes controlling zinc efficiency
2013
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Advisor: Prof. Dr. Saadet Büyükalaca ; Doç. Dr. Nedim Mutlu
Abstract (EN)
Zinc deficiency is a widespread problem reducing yield and quality of crop plants in Turkey like the world. This deficiency causes stunting, and leaf chlorosis reducing yield and quality of crops due in part to loss in photosynthetic capacity. It is important to improvement and use of molecular markers which linked to controlled to Zn efficiency for high Zn efficiency cultivars in Pepper(Capsicum annuum L.) Fort his aim; it was calculated to heridety of genes of controlled to Zn deficiency symptoms using , BC1, BC2 and F2 populations derived from C. annuum L. (Alata 21A) X C. frutescens L. (PI 281420) cross and also it was determined QTLs which linked to Zn efficiency using F2:F3 populations in pepper. As obtained results; genetic variance, additive variance, domianat variance and environment variance were calculated as 0,818, 0,226, 0,592 and 0,090, respectively. broad sense heritability, narrow sense heritability were calculated as (h2b) 0,906 and (h2n) 0,249, respectively. Also, it was calculated effect of additive gene as 28% and effect of dominant gene as 72% in total genetic variance. In addition, It was determined 12:3:1 genetic ratio as the best model for heridety of Zn deficiency symptoms as X2 analysis. Otherwise, controlled gene number to Zn deficiency symtoms was determined as 1,514 genes. It was obtained a genetic map with 929,6 cM lenght and 12 linkaje group using total 62 number polimorphic markers(31 SRAP, 19 SSR ve 11 RAPD primers) via JoinMap 4.1 program. Also, 41 linked QTLs with Zn efficiency for 9 characters were determined using F2:F3 populations at MapQTL.6 program. Obtained results show that it can be used to improvement of high Zn Effect pepper cultivars.
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Hasan Pınar
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Hasan Pınar (Doctorate thesis). Determination of heritability of zinc-efficiency in pepper (Capsicum annuum L.) and development of molecular markers linked to qtl/genes controlling zinc efficiency, 2013, Çukurova University, Biyoteknoloji Bölümü.
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