Effect of bioactive material-loaded bacteriocin-based nanofibers on the some quality parameters of rainbow trout (Oncorhynchus mykiss) fish

In this study, nisin, a natural antimicrobial bacteriocin, and curcumin, a natural bioactive substance, were loaded to polyvinyl alcohol-based nanofibers by electrospinning technique and nisin-curcumin loaded (NK) nanofibers with an average diameter of 172 nm were produced. Molecular characterization of the produced nanofibers was performed by ATR-FTIR spectroscopy. The rainbow trout fillets were then coated with nanofibers and the coated samples were stored at 4±2°C for 12 days. The effect of NK nanofibers was tested to limit the total number of mesophilic aerobic bacteria (TMAB), total lactic acid bacteria (TLAB) and total yeast-mold (TMK) in fish fillets during the preservation period. On the 4th day of the storage, the TMAB load of the control (K) group was 6.61 log cfu/g and the group coated with NK nanofibers was 3.28 log cfu/g. The TMAB load of the K group increased above 6 log cfu/g on the 4th day of the cold storage, while the TMAB load of NK group reached 6 log cfu/g on the 12th day of storage. During the analysis, a 3-4 logarithmic limitation of TLAB was determined with nisin and curcumin loaded nanofibers. All sensory characteristics of the K group were acceptable up to the 4th day of the case, while the sensory characteristics of the NK group were acceptable up to the 12th day of the case. The increase in color values (L*, a*, b*) of the K group was found to be higher than the NK group and it was determined that nanofibers preserved the color parameters of samples. This study demonstrated that nanofibers loaded with nisin and curcumin can be used effectively to improve the quality of fish fillets. Keywords: Bacteriocin, Curcumin, Electrospinning, Nanofiber, Nisin, Rainbow trout.