The research on the effect of chitosan on some immunologic parameters for applied Tilapia (Oreochromis niloticus) individuals

ABSTARCTMSc THESİSTHE RESEARCH ON THE EFFECT OF CHITOSAN ON SOMEIMMUNOLOGIC PARAMETERS FOR APPLIED TILAPIA (Oreochromisniloticus) INDIVIDUALSRozelin AYDINDEPARTMENT OF FISHERIESINSTITUTE OF NATURAL AND APPLIED SCIENCESUNIVERSITY OF ÇUKUROVASupervisor : Prof. Dr. İbrahim CENGİZLERYear 2006,Pages:29Jurry : Prof. Dr. İbrahim CENGİZLERProf . Dr. Necdet AYTAÇAssist. Prof. Dr. Aysel SAHANIn this research, effects of chitosan on non-specific defence mechanism wereinvestigated.The current study was used 50 mg/L, 100mg/L, 150 mg/L chitosan.Blood samples were collected at 1, 3, 7, 14 and 21 days. The current study wasdetermined leucocrit, NBT and phagocytic activity. The current study showed that atday 3 among the doses, all chitosan groups of leucocrit valuses were increased. Atday 7 and 21, the groups whic were 50 mg/L were increased in leucocrit values. Thechitosan groups which were 100 mg/L were increased in leucocrit values in all daysexcept at day 7 (p<0.05). The dose of 150 mg/L of chitosan were increased in allleucocrit values in all days except at day 3 (p<0.05). At day 1,3, 7, 14 and 21, amongthe doses, first and second doses were significant differences in NBT positive cellactivation values when to compare the control groups (p<0.05). At day 21, the valuesof phagocytic activity were significant differences (p<0.05). Between at day 1 and14, at 150 mg/L , the values of phagocytic activity were significant differences whento compare the control groups (p<0.05).Key words: Tilapia, Chitosan, NBT, Phagocytic activity, LeucocritII