The effect of intravitreal etanercept, cyclosporin A, triamsinolon asetonid on cytokines in experimental uveitis

Uveitis is often a chronic disease with debilitating consequences, such asprogressive vision loss and ocular discomfort and requires long-term medicaltherapy. Cytokines play an important role in the initiation of uveitic inflammation.Inthis study, The role of intravitreal etanercept, cyclosporin A and triamsinolonasetonid were aimed to assess on cytokines in experimental uveitis. All the same,Th17 and IL-23 were investigated on diagnosis and treatment.Five groups which were contained seven guinea pig were formed. Animals infour groups were immunised left eye with 1mg/ml conconavalin A. Eyes in group1(control) were not immunised and not given any treatment. After two weeksadministration of conconavalin A, eyes in group 2 (sham) were received single dose0.1ml saline intravitreally. In group 3 were installed single dose intravitreal 2 mg/0.1ml etanercept. In group 4 were received intravitreal 100 ?g/0.1 ml cyclosporin Aweekly. In group 5 were administered single dose 4 mg/0.1 ml triamsinolon asetonidintravitreally. After six month, all animal eyes were excised. Th17 immunostainingintensity and extent of retina and choroidea was assessed mild, modarate, severe and,IL-23 was evaluated by ELISA.Th17 immunostaining increased sham group than control and treatmentsgroups (p<0.001), treatment groups were similar each other (p>0.05). IL-23 levelwas higher in sham than control group ( p<0.001) and same with treatment group(p>0.05) by ELISA analyse IL-23 level was similar between treatment groups(p>0.05).Th17 and IL-23 caused and increased ocular inflammation. Administration ofetanercept, cyclosporin A and triamsinolon asetonid decreased Th17 immunostainingintensity extent but not effect IL-23 level.