Marine-derived phytase producer strain of aspergillus MF44 identification and determination of phytase production capaties

Besides the terrestrial habitats of marine fungi, sediment, algae, sponges and mangrove forests as they are very isolated from various marine habitats. This marine fungi isolated from marine habitats, new and different enzymes, secondary metabolites, constitute a rich source of natural compounds with biological activity and biodiversity. Futhermore, because of they are important features of biotechnological terms, increasing studies on marine fungi. In this study, strain of Aspergillus MF44, which isolated from a sponge in Mersin coast, were performed molecular and phenotypic tests for identifying species. As a result of this study is thought to be a new species isolates. Aspergillus MF44 isolate were determined that produced extracellular phytase enzyme, were made fermantation studies. Partial purification of the enzyme phytase enzyme with 80% acetone precipitation was carried out. After the 80% acetone precipitation, phytase was purified 2.5-fold with a recovery of %45,6 and its specific activity was up to 38,4 U/mg. Optimum temperature and pH values of enzyme were determined as 90°C and 5,0, respectively. It is determined that the phytase enzyme remained its activity of %69 at 90°C and end of the 1 hour. Fe+2, Ca+2, Co+2, PMSF, β-merkaptoetanol, hexane was found to reduce the enzyme activity. Phytase enzyme was partially purified and characterized with this study. Determination of these properties and have been found to have significant potential for industrial production.