Relations between castration and male and female rat's bladder wall, trigon and proximal urethra on endothelial, inducible and neuronal nitric oxide levels

Objective: To investigate the effects of castration to nitric oxide levels in lower urinary tract tissues (bladder, trigone and urethra), and then shedding light on clinical trials in patients undergoing castration to be associated with phosphodiesterase inhibitors. We aimed to illuminate testosterone significant role in the development of lower urinary tract symptoms and possible relationship between nitric oxide. Material and Method: In this study, a total of 32 albino Sprague Dawley rats were used. The different tissues which taken from ovariectomy, orchiectomy was performed and all the animals of the control group was left to special tube in which 10% formaldehyde found in label given code numbers and group names. Histopathological and immunohistochemical examination of the bladder and urethra taken to make tissue staining with nitric oxide antibodies were blocked after passed from routine procedures. Justified stained tissues were photographed and where appropriate nitric oxide antibodies cut in 4-5 micron sections. Results: In control and castrated rat groups significant results in histological and immunohistochemical staining of trigone of the bladder and body section in terms of eNOS and nNOS activity were obtained. There are no significant data in terms of iNOS. In control and orchiectomy rats groups made eNOS and nNOS activity in the proximal urethra for histological and immunohistochemical staining were obtained significant results. In female rats group tissue could be obtained due to the shorter urethra. Conclusion: In the study are statistically significant results were obtained in NO and NOS types of bladder and urethra were affected by castration. As a result, to take place in the treatment of overactive bladder or lower urinary tract symptoms, these studies should be supported by urodynamic data and then could continue to be a glimmer of hope on the way of treatment. NO mechanisms may be clinically important in maintaining normal urination and urinary incontinence. These mechanisms may be partly responsible for voiding dysfunction and urinary incontinence which developed after pelvic surgery or spinal injuries. Thus, in this cases if further studies supports these findings the treatment can be possible with NOS agonists or NO donors.