The effect of bromopropylate organophosphate pesticide on the some metabolites and mitochondrial electron transport enzymes in Trichoderma harzianum

In order to identify the aspects of organophosphate pesticide bromopropylate concentrations effect on electron transport system (ETS) enzymes; succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), tricarboxylic acid (TCA) cycle metabolites; citrate, ?-ketoglutarate and fumarate and glycolysis last metabolite pyruvate level as well as lipid peroxidation (LPO) levels of eukaryote model, Trichoderma harzianum were investigated with respect to incubation period. The mitochondrial respiratory system is one of the most important systems in the central metabolism and includes glycolysis, TCA and ETS.In the present study, the SDH activities of T. harzianum increased with the rises in bromopropylate concentration up to 2.5 mg/L. In contrast to SDH, COX activities increased respect to increases in the bromopropylate concentration by showing induction effect of the pesticides on COX enzyme. As an end product of glycolysis, pyruvate levels increased markedly up to 2.5 mg/L of bromopropylate. Although the intermediate of the TCA cycle, intracellular citrate levels rose with the increases in bromopropylate concentration up to 7.5 mg/L, the other TCA cycle intermediates, and intracellular ?-ketoglutarate and fumarate levels increased up to 5.0 mg/L of bromopropylate. However, the variations of ATP, ADP and AMP levels showed positive correlation with ?-ketoglutarate, fumarate levels. The results may indicate that the pesticides have been reported to influence intracellular nucleotide phosphate concentration with respect to TCA cycle and oxygen uptake. As an indicator of membrane damage, LPO levels enchanced with respect to increases in bromopropylate concentrations and incubation periods which is probably due to leakage of electrons from ETS.