Effect of lycopene from Solanum lycoericum in cell line and cell death

The aim of this study was to evaluate the effect of the lycopene extract from Solanum lycoericum (tomato) on the bacterial and proliferation of lymphocytes and three cancer cell lines (Human breast cancer AMN3, hepG2, and MEF. The first study affects the antimicrobial activity of lycopene extract. The second assay studies effect of lycopene extract on lymphocyte division in human blood. The third assay including the effect of lycopene extract Solanum lycoericum on the growth of tumor cell lines AMN3, HepG2, and MEF was done by using cytotoxic assay. The results show that the extraction lycopene extract revealed cytotoxicity on different cells line, and this effect depends on concentration and cell line types. In gene expression studies, the results showed the downregulation of the genes Hsp60, and Hsp70 in AMN3 and HepG2 cancer cell lines. While the gene expression of apoptosis genes, the Caspase 8 gene was high regulation for all used cancer cell lines. All results showed that the Lycopene extract of Solanum lycoericum (tomato) induced cell death by mitochondrial Intrinsic pathway after 24 hrs of exposure.