Investigation of the distribution of allele frequencies for 15 STR loci of the population of elazig and neighboring provinces

The purpose of this study is to examine the distribution of 15 STR loci allele frequencies of the populations of the city Elazig and surrounding cities (Malatya, Tunceli, Bingöl, Diyarbakır). It?s considered that, this study will contribute in determining the similarities and differences toward the gene pool frequencies of the population of the city Elazig and surrounding cities, and the other regional and foreign gene pool frequencies and their overall evaluation criteria. The allele distribution frequencies of the genotype data which also will be determined, constitute an important data resource for studies related with forensic science in being able to identify persons. This study was performed at the Firat University, Medical Faculty, Forensic Medicine Department, DNA Laboratory on 250 individuals in total, biological samples were obtained from these individuals who weren?t relatives, and were members of the populations of the city Elazig and surrounding cities (Malatya, Tunceli, Bingöl, Diyarbakır), 50 individuals participated from each of these cities. A RTA Blood Kit and genomic DNA?s were derived from the samples. The gene regions of the required loci of the isolated DNA?s, were multiplied with the use of Amp F / STR Identifier PCP Amplification Kit (Applied Biosystems, Foster City, CA, USA). The electrophoresis of PCR items was carried out with the ABI 310 sequence instrument, and the results were analyzed with GeneMapper Software Version 3.2. The results obtained were evaluated with PowerStarts v.1.2 and Arlequin Software 3.11, and after that the examined allele densities, other statistic data of the populations and the genetic distances of the populations inter se were determined. Similar studies were carried out on 15 STR loci in Turkey (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA), but there wasn?t a study which evaluated Elazig and surrounding cities together. The continuity force values of this study; the highest loci determined in Elazig D2S1338 (0,960), in Malatya D2S1338 (0,953), in Bingöl D2S1338 (0,964), in Tunceli D18S51 (0,952), and in Diyarbakır D18S51 (0,962), the lowest loci determined in Elazig TPOX (0,808), in Malatya CSF1PO (0,829), in Bingöl TPOX (0,863), in Tunceli D5S818 (0,833), and in Diyarbakır D5S818 (0,817). It?s also observed that, the most distant genetic relation exists between Elazig and Tunceli when the populations were inter evaluated in duos. As a result, it?s considered that obtaining allele frequencies of 15 STR loci will utilise the comparison of these data and previous studies, and the evaluation of the phylogenic relations. Key words: Short tandem repeat, Allele, Elazig and surrounding cities