Protective effect of resveratrol on fumonisin b1-induced hepatotoxicity in mice

In this study, the effect of resveratrol against fumonisin B1 (FB1) induced liver toxicity in mice was investigated. For this purpose, 40 BALB/c mice were randomly assigned to control, FB1, resveratrol and FB1+resveratrol groups. Control group was received saline intraperitoneally for 14 days, FB1 group was received 2,25 mg/kg FB1 intraperitoneally every other day for 14 days, resveratrol group was received 10 mg/kg resveratrol intraperitoneally for 14 days, FB1+resveratrol group were administered intraperitoneally 2,25 mg/kg FB1 every other day and 10 mg/kg resveratrol daily for 14 days. At the end of the study, mice were euthanized and blood and liver tissue samples were taken. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total sialic acid (TSA) analyzes were performed in serum samples obtained. Total antioxidant status (TAS), total oxidant status (TOS) analysis of liver tissue samples and histopathological examination of these tissue samples were also performed. AST, ALT, TSA results of FB1 group were higher than control group (p<0.05). In the FB1+resveratrol group, AST and ALT levels were found to be low compared to the FB1 group, but these levels were not different from the control group. TSA values in FB1 group were not different from FB1+resveratrol group but FB1 + resveratrol group values were not also different from those of control group. TAS values in the liver of FB1 group were lower than in control and TOS values were higher than in control (p<0.05). The TAS and TOS values of the FB1 + resveratrol group were not different from those of the control group. Histopathological examination revealed that FB1 causes pathological findings characterized by hyperemia and infiltrations in the liver of FB1 group. In addition, in this group, some hepatocytes showed megalocaryosis in the nuclei. There were no pathological findings in the control, resveratrol and FB1+resveratrol groups. According to the biochemical and histopathological findings, resveratrol has protective effect against liver damage and oxidative stress caused by FB1. In addition, the increase in serum total sialic acid levels can be used as a biomarker for FB1 toxicity. It was concluded that resveratrol can be used as a therapeutic agent against the toxic effects of FB1.