Effects of fluvalinate sub-lethal concentration on oxidative stress and antioxidant enzymes in some tissues of guppy (poecilia reticulata peters, 1859)

The purpose of the study is to research effects of sublethal concentrations of Fluvalinate, which is a synthetic prethyroid, on antioxidant enzymes activities and lipid peroxidation in female guppies (Poecilia reticulata Peters, 1859). Guppies were exposed to sublethal doses of Fluvalinate [(96 h, LC50:0,026 μg/L), (0.0033, 0.0065 ve 0.063μg/L)] in four different timeframes (0, 24, 48 and 72 hours) during 96 hours. After the experiment, the fish were anesthetized with clove oil (%85-95 eugenol (4- ally-methoxyphenol-C10H12O2), %5-15 isoeugenol ve methyleugenol). Tissues were taken with the help of stereo microscopes. To determine oxidative effects of Fluvalinate, Superoxide Dismutase (SOD), Glutathione Peroxidase (GSH-Px), Catalase enzyme activities (CAT), levels of Malondialdehyde (MDA) on muscles and gill tissues were detected through spectrophotometric methods. Subletal fluvalinate doses caused a significant decrease in antioxidant activity (SOD, GSH-Px and CAT enzyme activities) compared to the control group, MDA levels increased significantly compared to the control group (p ≤ 0.05). In this study, fluvalinatenin has oxidative stress on Poecilia reticulata Peters, 1859 and can be used as a biomarker in the determination of toxicity.