A compact system design and antimicrobial analysis of the produced nanocomposite with visible light sensitive Ag carrier - CMC/MA/ODA-MMT activating agnp production

The use of silver nanoparticles as antimicrobials has been increasing in recent years. For this reason, various silver (Ag) carrier polymer structures are formed and silver nanoparticle (AgNP) formation is encouraged by different methods. The aim of this thesis is; The effect of visible light in three different wavelengths (blue, green, red colors) on the nanoparticle formation in Ag-carrying CMC / MA / ODA-MMT nanocomposite, a low-cost system has been designed for the investigation and monitoring of this activation process. In the literature, no system has been found that can provide activation at 3 different visible wavelengths and show the activation occurred with data. For this reason, a compact and low cost handmade system was designed, activated with visible light in three different wavelengths (green, blue, red colors) for the formation of nanoparticles in Ag-carrying CMC / MA / ODA-MMT nanocomposite, and this activation was carried out with PIN photodiodes were detected and activation times were observed. With the designed system, it was observed that the potential difference was determined during the formation period of AgNPs, while the potential difference was set at the end of the activation process. The potential difference-time curves of the samples activated by blue, green and red LEDs after the nanocomposite activation were analyzed. It was determined that the potential difference taken from all LEDs during activation decreased with the exponential curve against time, and it was observed that the potential difference was set at the end of the activation process. After the activation of the nanocomposite, TEM-SEM analyzes and antimicrobial tests of the samples activated by each wavelength were performed and the samples were compared in terms of nanoparticle sizes and antimicrobial activities. When nanoparticle sizes of AgNP / CMC / MA / ODA-MMT with different wavelengths are activated compared to the energy values of LEDs; It has been determined that the AgNP size of the LED with high energy is small (the average nanoparticle size is 4-6 nm for the sample activated by the blue LED, 6-8 nm for the sample activated by the green LED and 12-14 nm for the sample activated by a red LED). It has been determined that the wavelength, i.e. the energy, is effective on the activation time. It was measured that the AgNP activation time of the LED with the highest energy was shorter (average activation times were 12 minutes for the sample activated by the blue LED, 18 minutes for the sample activated by the green LED, and 24 minutes for the sample activated with a red LED, respectively). To observe antimicrobial activity and concentration (2,5 mg / ml, 0,5 mg / ml, 0,1 mg / ml, 0,05 mg / ml, 0,01 mg / ml, 0,005 mg / ml, 0,001 mg / ml A disk diffusion test was performed to observe the effect on antimicrobial activity (0 mg / ml). In the antimicrobial activity of AgNP / CMC / MA / ODA-MMT where the disk diffusion test (Gram negative E.coli 25922, P.aeruginosa 27853, Gram positive E.faecalis 29212 bacteria and Candida albicans 10231 as fungi) was performed, the nanoparticle sizes of the samples were approximately the same. It has been observed that nanoparticle size (wavelength) does not make a significant difference. It was observed that samples with high AgNP / CMC / MA / ODA-MMT concentrations had high antimicrobial activity. For this study, it was observed that the concentration of AgNP / CMC / MA / ODA-MMT was effective in antimicrobial activity. Based on the results of the study, it can be said that an easy to use, compact and low cost antimicrobial system was designed to produce antimicrobial nanomaterials in a controlled stage and harmless to the environment in this period, which was struggling with Covid-19.