Metallic nanoparticle synthesis in halophilic archea

It is aimed to synthesize potential chromium, silver, zinc and nickel nanoparticles with halophilic archaea, within the scope of the thesis. The potential nanoparticle producer candidate isolates were selected by screening a total of 113 haloarcheal strains isolated from saline soil and brine samples and standart strains for their ability to grow in complex agar and broth media containing 20% NaCL and different metal salts (0.5 and 1 mM). Metallic nanoparticles were produced by adapting the selected isolates to metal salts at increasing concentrations up to 1.5 mM in broth medium. The characterization of nanoparticles produced intracellularly under agitated conditions was determined by XRD and SEM-EDS. XRD analyzes of biosynthesized silver nanoparticles showed that they have face-centered crystal structures. SEM-EDS analaysis revealed the formation of well-dispersed nanoparticles and the presence of elemental form of each metal was confirmed. To the best of our knowledge, this is the first report on the synthesis of chromium, nickel and zinc nanoparticles by haloarchaea from environmentally friendly biogenic systems, apart from physical and chemical methods.