Analysis of in vitro biological activities and bioactive composition of helichrysum pallasii (sprengel) ledeb plant

Analysis of In Vitro Biological Activities and Bioactive Composition of Helichrysum pallasii (Sprengel) Ledeb. Plant, Van Yuzuncu Yil University, Institute of Health Sciences, Department of Basic Pharmaceutical Sciences, Master Thesis, Van, 2020. This study aimed the investigation of in vitro biological activities (antioxidant and enzyme inhibition) and bioactive composition of various extracts (ethanol, infusion, and decoction) obtained from leaf organs of Helichrysum pallasii plant which has been traditionally used in the treatment of various disorders such as kidney diseases, stomachache, asthma, diarhhea, hepatitis, and diabetes in Van province. Laboratory analysis showed that ethanol-based extract showed superior antioxidant (FCR: 103 mg Gallic acid Eq./g; FRAP: 2205 µmol Fe2+/g; ORAC: 2540 µmol Trolox Eq./g; DPPH: IC50-0.58 mg/ml; CUPRAC: IC50-0.37 mg/ml; Phosphomolybdenum: IC50-1.34 mg/ml ve metal chelation: IC50-1.42 mg/ml) and enzyme inhibition (Acetylcholinesterase: IC50-1.49 mg/ml; Butyrylcholinesterase: IC50-1.98 mg/ml; Tyrosinase: IC50-0.68 mg/ml; Alpha-amylase: IC50-2.09 mg/ml; Alpha-glucosidase: IC50-0.51 mg/ml; and Pancreatic lipase: IC50-42.5 µg/ml) activities and contained higher amounts of phenolic (Chlorogenic acid isomers and rutin) and fatty acid (Palmitic, linoleic, and linolenic acids) compounds than traditional preperations (infusion and decoction). Data obtained from the current study shows leaves of Helichrysum pallasii plant as a source of phytochemicals with strong antioxidant and enzyme inhibitory properties and suggesst it's candidate in research and development of biotherapeutic agents studies.