Creation of 3D structures with hydrogel bioinks and investigation of effects on angiogenesis in chick chorioallantoic membrane mode

Based on the literature research, it was observed that the angiogenesis process was not followed when the hydrogel print extracted from the bioprinter worked with the chorioallantoic membrane model (CAM) and was integrated in the interaction with the hydrogel in the chorioallantoic membrane and angiogenesis development experiment. The aforementioned model should enable quantitative measurement of neovascularization, so that the physical formation between the chorioallantoic membrane model (CAM) and the hydrogel printed by the bioprinter can be monitored. When blood vessel formation is evaluated, it is a complex multi-step process in which more than one cell type and large number of molecules participate. When the angiogenesis process is monitored, it is controlled depending on the balance between the molecules that affect the process positively and negatively. This is called the "angiogenic switch". Based on the literature research, hydrogels are a network of cross-linked homo or copolymeric systems that can absorb a high amount of water and create a "swelling" feature. They are three-dimensional polymeric networks that cannot dissolve in water and swell, that is, they can take most of the water into the structure. Gelatin, agar and alginates are in the group of both natural and synthetic polymers. The prints taken from the 3D Printer of the hydrogels containing alginate produced within the scope of the project were created by crosslinking by spraying method. When we look at the literature research, it is observed that the cross-linking is generally done by UV light; Steam spraying method with CaCl2 was tried and observed. Cura program was used while modeling hydrogels. Thanks to this program, 3D hydrogel production was realized in the desired geometry and dimensions. This interface also allows the positioning of the mechanism for transferring solutions to the syringe tip. The chick embryo chorioallantoic membrane model (CAM), which has a dense vascular structure and is frequently used in angiogenesis studies, is a useful in vivo system. It is used as an alternative to experimental animals in our Angiogenesis Research Laboratory. This model is known as the CAM (chick chorioallantoic membrane) model, which is based on the use of eggs with embryos. The CAM model is an appropriate study model that is frequently used in angiogenesis studies. In the laboratory environment, the angiogenesis process was observed physiologically in the model by utilizing the biorepressible feature of the hydrogel to be formed by editing chemical prescriptions. Within the scope of the project, the change and effect of the vein structures in the CAM model of the hydrogel, which was developed by trial methods, were examined. In project scope; How angiogenesis, which is an important parameter in vessel formation, is affected by progesterone, which is frequently used in tissue scaffolding, was investigated by means of the chorioallantoic membrane model (chorioallantoic membrane-CAM) in chick eggs. The effect of progesterone in different combinations on eggs divided into 3 different groups was investigated. A general result was obtained by comparing each group with the control group and other groups.