High performance thin-layer chromatographic method applications for qualitative and quantitative analysis of flowering aerial parts of hypericum perforatum l. and their implementations for some hypericum species and products

Hypericum species (Hypericaceae) have been traditionally used as remedial drugs for ages across the world and in Turkey. Among Hypericum L. species H. perforatum L. is recognized as the medicinally most important member. A vast number of experimental studies have been published so far and pharmacological activities have been mostly attributed to its hyperforin (phloroglucinol derivative), hypericin (naphthodianthrone derivative) and/or hyperoside (flavonoid derivative) contents. In the European Pharmacopoeia, these three marker components in "Hyperici herbae extractum siccum quantificatum" were described for qualitative (by TLC method) and quantitative analysis (by HPLC method). On the other hand, HPTLC, as a new analytical tool, has become increasingly used as a suitable technique in analysis of herbal drugs. Within the context of this thesis, three HPTLC methods were developed and validated on HPTLC plates coated with silica gel 60 F254 for qualitative and quantitative analysis of hyperforin, hypericin and hyperoside in the flowering aerial parts of H. perforatum as an alternative to TLC and HPLC methods stated in the European Pharmacopoeia. Three different mobile phases were used in HPTLC analysis; for hyperforin analysis n-hexane-ethyl acetate (8:2); for hypericin analysis toluene-chloroform-ethyl acetate-formic acid (8:5:3.5:0.6); and for hyperoside analysis ethyl acetate-formic acid-acetic acid-water (15:2:2:1). Then the developed and validated HPTLC methods were applied on different Hypericum species and practiced to test their applicability for the quality analysis of several Hypericum samples and oily macerates purchased from herbal shops based on the concentrations of these three marker components.