Investigation of pro12ala polymorphism in the peroxisome proliferator-activated receptor-gamma gene in cancer cell lines

Single nucleotide polymorphisms (SNPs) are the variations in single nucleotide in the DNA sequence. They are the most common genetic variations in genome. It is known that SNPs may have effects on gene expression and function. The Pro12Ala polymorphism is the change of proline amino acid to alanine amino acid by the conversion of cytosin(C) to guanine (G) at 34th nucleotide of the 12th codon in the Peroxisome Proliferator Receptor Gamma (PPARγ) gene. Several recent studies have shown that Pro12Ala polymorphism may be associated with many diseases, such as obesity, diabetes, and some types of cancer. At the cellular level, there were no studies investigating the relationship between cancer and Pro12Ala polymorphism. In this study, Pro12Ala polymorphism in PPARγ gene was investigated in cancer cell lines and normal cell lines via using restriction fragment length polymorphism (RFLP) method and the effect of Pro12Ala polymorphism on PPARγ mRNA expression was investigated via using Real Time Polymerase Chain Reaction method. In conclusion; amongst 13 cell lines that are screened, Pro12Ala polymorphism was detected in only AGS and Caki-1 cell lines. PPARγ mRNA expression of these cell lines was found to be lower than PPARγ mRNA expression of cell lines without Pro12Ala polymorphism such as MCF10A, SK-BR-3, MDA-MB-453, MDA-MB-458 cell lines.