Isolation and structural determination studies from Cephalaria taurica Szabo. species

In this project, we planed to investigate biologically active natural compounds from Cephalaria taurica Szabo (Caprifoliaeceae) species which is an endemic plant to our country. The plant material was identified and collected in flowering stage. The air dried material was grinded made ready for chemical study. Plant material was extracted with MeOH, n-BuOH: H2O and hexane solvent and solvent systems. VLC, MPLC, CC and TLC methods and MeOH: H2O, CHCl3: MeOH: H2O solvent systems were used for isolation and purification respectively. A series of advanced spectroscopic methods (1D- and 2D-NMR, FT-IR, UV and GC-MS) were used for structurel identification of the pure isolated compounds obtained by choramagraphic and chemical proseses. As a result, two new triterpenoid saponins with eight sugar moieties (1 and 2) and two new prosapogenins (1a and 2a) were chemically investigated and their biological activities were tested for the first time in Cephalaria genus.