Effect of clove oil on stocking density in the transportation of goldfish (arassius auratus)

In this study, optimal concentration of clove oil for the anesthetization of gold fish (Carassius auratus) with an average weight of 4.16±0.61g at 30 min (minutes) and 15 h (hours) periods was determined. Besides, the optimal stocking rate for the transportation of fish in oxygen compressed nylon bags for 15 hours and the effect of clove oil on the stocking rate was determined. Depending on the concentrations and application time of clove oil, induction time ranged from 2 to 11 min while recovery time varied between 5 and 17 min. Depending on the concentrations and application time of clove oil, respiratory rate of fish increased from 73/min to 104/min with the addition of clove oil in the anesthetic-free water. 6-12 µL/L for light anesthesia and 18-24 µL/L for deep anesthesia at a 30 min period, 6-9 µL/L for light anesthesia and 12-18 µL/L for deep anesthesia at a 15 h period are determined concetrations of clove oil that can be used safeley. The toxic concentrations of clove oil were determined as 42 µL/L for 30 min application, 21 µL/L for 15 h application. Adding clove oil to the transport water had no effect on the survival rate of the fish during the 15 h transport period.When transporting juvenile goldfish in 3/4 oxygen compressed nylon bags for 15 h, it is possible to stock 80 or 100 fish/L (332 or 416 g /L) with 98-99% survival rate.