Determination of carbapenemase production by BD phoenix CPO method in karbapenem resistant Klebsiella pneumoniae and Escherichia coli isolates

Aim:Carbapenemase-producing organisms (CUO) are Gram-negative bacteria that are resistant to most, sometimes all, antibiotics with their carbapenemase enzymes. Rapid and accurate detection of carbapenemases is essential for the early detection and prevention of resistant nosocomial infections. Material and Method:The study included 72 isolates, 64 containing Oxa-48, 6 NDM, 2 NDM+Oxa-48 and 16 isolates without carbapenemase, isolated from various clinical samples sent to the bacteriology unit of Dicle University Hospital Central Laboratory between January 2020 and December 2020. Carbapenemase enzyme groups were investigated in isolates with the BD Phoenix CPO Detect (Becton Dickinson, USA) kit. Results:The compatibility of the CPO Detect test with PCR was 95.3% for Oxa-48 carrying isolates and 100% for those with the NDM gene. The negative predictive rate of the test was 88.8% in all isolates, 83.3% in K. pneumoniae isolates, and 100.0% in E. coli isolates. Conclusion:The usage of the CPO Detect test, which can detect carbapenemase enzymes , in routine diagnostic laboratories will contribute to the early diagnosis and correct treatment of resistant bacteria. Key Words:CPO Detect, Carbapenemase, Oxa-48, Klebsiella pneumominae, Escherichia coli