Purufication of catalase from the walnut (Juglans regia)
Is this your thesis?
This record came from a bulk archive import. If it’s yours, link it to your profile.
Abstract (EN)
In this study, we aimed to purify first time catalase enzyme (E.C.1.11.1.6) from walnut (Juglans regia ), to determine kinetic parameters (Km, Vmax), thermal and storage stabilities of the purified catalase. In the partial purification of catalase enzyme homogenization, ultracentrifugation and PD-10 (Sephadex G-25M) column chromotography steps were used. The optimal pH value of catalase was 8.0 and its optimal temperature was 25°C . Thermal stability of catalase at 25 °C compared to 40 °C and 4 °C in storage stability was higher than 25 °C. Km and Vmax values were 10,4 mM and 159,4 U/mg prot. , respectively. It was determined that the enzyme had 4 equal subunits each weighting 53 kDa and had a total having molecular weight of 212 kDa.
Author
Çağlar Akar
How to Cite
Çağlar Akar (Master Thesis). Purufication of catalase from the walnut (Juglans regia), 2015, Çukurova University.
Keywords
License
Tüm Hakları Saklıdır
This work is shared under the specified license terms.
More theses from Çukurova University
- The effects of collaborative video-blog projects on Turkish EFL students' linguistic and digital literacy skills(2025)
- An investigation of violent and nonviolent adolescent' families in terms in terms of family fuctioning, anger and anger expression(2006)
- Adolescents who have single parents family and full family were compared in respect to their life satisfaction and quality of life(2009)
- Credit risk management in banking sector: An application of variables determining credit risk in Turkish banking sector(2011)
- Investigation of psychological symptom levels in adolescents according to gender and family functions(2013)
- Assessing morphological and genetic diversity among traditional African eggplant landraces and detecting salt tolerance and anther culture performance of selected accessions(2022)